品牌：BioLife Solutions

貨號：PART No. 210102

容積：VOL. 100mL

型號：CryoStor CS10

BioLife Solutions CryoStor CS10細胞DMSO凍存液210102訂購信息

英文名：BioLife Solutions CryoStor CS10 Freeze Media

中文名：BioLife Solutions CryoStor CS10 細胞DMSO凍存液

BioLife Solutions CryoStor CS10細胞DMSO凍存液210102 代理銷售歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司  ：152 1681 4001。紅榮微再-客服: 316 808 6348  經銷商專員

BioLIfe Solutions CryoStor CS10細胞DMSO凍存液產品系列

CryoStor®, a series of cell-specific, optimized freeze media, is designed to prepare and preserve cells in ultra low temperature environments (-70ºC to -196ºC); CryoStor®, pre-formulated with DMSO, provides a safe, protective environment for cells and tissues during the freezing, storage, and thawing process. Through modulating the molecular-biological response to the cryopreservation process, CryoStor® provides for enhanced cell viability and functionality while eliminating the need for serum, proteins or high levels of cytotoxic agents.

各種規格的BioLIfe Solutions CryoStor 細胞DMSO凍存液

BioLife Solutions CryoStor®是BioLife公司特別優化的細胞凍存液，在極低溫度 (-70ºC to -196ºC)下準備和保存細胞。預混DMSO，為細胞和組織的冷凍、儲存和解凍過程提供安全的保護環境。CryoStor®通過調控凍存過程的分子生物學反應，無血清、蛋白或具有高細胞毒性的試劑，就能增強細胞活力和功能。

BioLife Solutions CryoStor CS10細胞凍存液特點

用于細胞和組織的高級生物保存液：

預混DMSO

無血清、無蛋白

USP級高質量成分、cGMP級生產、FDA master文件

滅菌、內毒素和細胞功能檢測

BioLife Solutions CryoStor CS10 細胞DMSO凍存液cGMP級生產

BioLife Solutions CryoStor CS10 Freeze Media 使用與凍存方法

CryoStor® Usage and Cryopreservation Protocol

細胞準備

1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)

2) Centrifuge cells to obtain cell pellet

3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.

加CryoStor凍存液

4) ISOLATION: Add cold (2-8°C) CryoStor®

a. Cell concentrations: 0.5-10 x 10⁶

 cells/ml for routine cell culture protocols (higher [cell] possible).

b. DMSO is pre-mixed in CryoStor® - no additives are necessary.

5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes

6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)

a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.

b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.

c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.

d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.

BioLife Solutions 儲存

7) STORAGE: Place samples into storage

a. Store samples at liquid nitrogen temperatures (below -130°C).

b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).

細胞復蘇解凍

8) THAWING: Thaw samples quickly in a 37°C water bath

a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.

b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.

Passive thaw is not recommended.

9) Dilute cell/CryoStor® mixture immediay with culture media

a. Dilution procedure can be preformed in a single step.

b. The dilution media should be between 20°C and 37°C.

c. A dilution ratio of 1:10 (sample to media) or greater is recommended.

10) Plate cells in appropriate configuration

11) Place cells into culture conditions or utilize immediay

12) Viability assessment 24-hours post-thaw*

BioLife Solutions Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.

*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.

Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.

Visual inspection of adherent cells and cells “floating” in the media is also recommended.

BioLife Solutions CryoStor CS10細胞DMSO凍存液210102 代理銷售歡迎您致電

重慶市華雅干細胞技術有限公司 ， ：

重慶市華雅干細胞技術有限公司以“專注干細胞研究，提升干細胞品質”為發展理念，專業從事干細胞基礎研究、干細胞再生醫學臨床應用研究及干細胞技術服務。