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Anti-CD11b [M1/70] 抗體 (ab8878)產品信息:
Rat monoclonal [M1/70] to CD11b
Reacts with: Mouse, Rabbit, Human
The hybridoma was formed by the fusion of mouse myeloma NS1 cellswith spleen cells from rats immunized with B10 mouse spleen cells enrichedfor T lymphocytes.
Properties
Concentration 100 µg at 1 mg/ml
Applications
Our Abpromise guarantee covers the use of ab8878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
IHC-Fr | IHC-Fr: Use at an assay dependent concentration. PubMed: 20962222 |
ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
Flow Cyt | Flow Cyt: Use at an assay dependent concentration. Each labeling step was carried out for 30 min at 0-4°C in mediumcontaining 0.01 M NaN3. Cells (5 x 107/ml) were incubated with 50 µl M1 monoclonal antibody or irrelevant monoclonal antibody, R4/18.2, as control in the first step, washed, suspended in 50 µl of FITC-F (ab')2 anti-rat IgGin the second step, and washed through a layer of fetal calf serum. Can also be used in cytotoxicity and binding assays. |
WB | WB: Use at an assay dependent concentration. PubMed: 20600810 |
Target
Database links
Alternative names
Anti-CD11b [M1/70] antibody images
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
ICC/IF image of ab8878 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8878 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Anti-CD11b [M1/70] antibody (ab8878)
Overlay histogram showing Human macrophages stained with ab8878 (blue line). Cells were incubated with human immunoglobulin for 30 min at 4°C to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8878, 20 µg/mL) for 30 min at 4ºC. The secondary antibody used was FITC mouse anti-rat IgG2b (gamma chain) (ab99671) at 1 µg/mL for 30 min at 4ºC. Isotype control antibody (black line) was Rat IgG2b [RTK4530] (ab18541) used under the same conditions.
Flow Cytometry - Anti-CD11b [M1/70] antibody (ab8878)
Overlay histogram showing Human macrophages stained with ab8878 (red line). Cells were pre-incubated in Human AB serum (10%) for 20 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 0.1µg/1x10^6 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Frozen sections) - CD11b antibody (ab8878)This image is a courtesy of Anonymous Abreview
ab8878 staining CD11b in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with 5% BSA for 1 hour at 250C was performed. The sample was incubated with primary antibody (1/100) for 12 hours at 40C in PBS. A Cy3®-conjugated donkey polyclonal to rat IgG was used at dilution at 1/500 as secondary antibody. Red staining in the image represents Cy3-CD11b, green - FITC-BrdU and blue is DAPI staining.Immunohistochemistry (PFA perfusion fixed frozen sections) - CD11b antibody [M1/70] (ab8878)This image is courtesy of an Anonymous Abreview.
ab8878 staining CD11b in Mouse brain tissue by Immunohistochemistry (PFA perfusion fixed frozen sections). The sections were perfusion fixed with paraformaldehyde prior to blocking with TNB blocking buffer for 1 hour at 24°C. The primary antibody was diluted 1/100 in TNB blocking buffer and incubated with the sample for 24 hours at 4°C. An HRP Polymer-conjugated Goat anti-Rat polyclonal was used undiluted as the secondary antibody. Detected using a Streptavidin-Alexa 546 conjugate.
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