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Anti-ATP5O 4C11C10D12 antibody （ab110276）抗ATP5O 4C
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產品型號：
 ab110276
產品報價：
產品特點：
 Anti-ATP5O [4C11C10D12] antibody (ab110276)抗ATP5O [4C11C10D12]抗體
貨號：ab110276
廠家：Abcam公司
規格：100ug
保存溫度：-20℃ 和 2～8℃
貨期：現貨2～5天，期貨2-3周
  ab110276Anti-ATP5O 4C11C10D12 antibody （ab110276）抗ATP5O 4C的詳細資料：

Anti-ATP5O [4C11C10D12] antibody (ab110276)抗ATP5O [4C11C10D12]抗體產品信息：

  • Product nameAnti-ATP5O [4C11C10D12] antibodySee all ATP5O primary antibodies ...
  • Description

    Mouse monoclonal [4C11C10D12] to ATP5O

  • Tested applicationsICC/IF, WB, Flow Cyt, IHC-P more details
  • Species reactivity

    Reacts with: Cow, Human, African Green Monkey

  • Immunogen

    Recombinant mature Human ATP5O.

  • Positive control
    • Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C. Do not freeze.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: HBS

  • Concentration 100 µg at 1 mg/ml

  • PurityIgG fraction
  • Purification notesNear homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality Monoclonal
  • Clone number4C11C10D12
  • IsotypeIgG1
  • Light chain typekappa
  • Research Areas
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex V
    • Metabolism
    • Types of disease
    • Cancer

Applications

Our Abpromise guarantee covers the use of ab110276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

ApplicationNotes
ICC/IFICC/IF: Use a concentration of 0.5 - 1 µg/ml. heat-induced antigen-retrieval improves signal
WBWB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa.
Flow CytFlow Cyt: Use a concentration of 1 µg/ml.
IHC-PIHC-P: 1/500.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similaritiesBelongs to the ATPase delta chain family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    • Entrez Gene: ATP5O Cow
    • Entrez Gene: 539 Human
    • Omim: 600828 Human
    • SwissProt: P13621 Cow
    • SwissProt: P48047 Human
    • Unigene: 49172 Cow
    • Unigene: 409140 Human

    • Alternative names

      • ATP synthase O subunit mitochondrial precursor antibody
      • ATP synthase subunit O antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody

    Anti-ATP5O [4C11C10D12] antibody images

    • Western blot - ATP5O antibody [4C11C10D12] (ab110276)

      Western blot - ATP5O antibody [4C11C10D12] (ab110276)

      All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 0.5 µg/ml
      Lane 1 : Human heart mitochondria at 5 µg
      Lane 2 : Bovine heart mitochondria at 1 µg
      Predicted band size : 23 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)

      Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

    • Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)

      Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)

      Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

    • Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)

      Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)

      HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

    • Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

      Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)

      All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 1 µg/ml
      Lane 1 : HDFn (human) cell lysates at 20 µg
      Lane 2 : COS7 (monkey) cell lysates at 20 µg
      Lane 3 : H4IIE (rat) cell lysates at 20 µg
      Lane 4 : MEF (mouse) cell lysates at 20 µg
      Lane 5 : bovine heart mitochondria lysates at 5 µg
      Predicted band size : 23 kDa

      Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

    公司介紹：

        Abcam位于英國的劍橋科學園，成立于1998年，專門生產和分銷研究型抗體。主要創立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網上抗體公司，理念是Abcam銷售質量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務，及高效率的顧客和服務。我們的在線目錄(ladywindows.cn) 已有差不多100,000種抗體和試劑，并不斷添加，供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設有分公司。

        Abcam 一直致力加強產品線，為使研究員更容易找到蛋白質研究試劑產品，在2011并購了美國的MitoSciences公司，加強了免疫分析方面的產品供應；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應商。

        Abcam 的 目標是給世界上的抗體建立zui大的在線目錄，為各地科學家提供*產品，成為各國科學界的重要伙伴。我們為所有產品提供來使客戶獲得預期的結 果。為提供高質量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應用和物種中檢測每種抗體。我們相信誠信才是上策，在我們的上會發布有關我們 每一種產品的盡可能多的信息。

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    重慶市華雅干細胞技術有限公司

     
    產品相關關鍵字： ab110276 ATP5O ATP合酶O單元線粒體前體抗體 ATPO antibody ATP合酶亞基O抗體
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