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Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)產品信息:
Rabbit polyclonal to Histone H3 (tri methyl K36) - ChIP Grade
Reacts with: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Schizosaccharomyces pombe, Zebrafish, Rice, Trypanosoma brucei
Predicted to work with: Plants
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, tri methylated at K36.
Read Abcam's proprietary immunogen policy
(Peptide available as ab1785.)
For detection of methylated histone H3
Properties
Concentration 100 µg at 1 mg/ml
Applications
Our Abpromise guarantee covers the use of ab9050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
CHIPseq | CHIPseq: Use at an assay dependent dilution. PubMed: 19581485 |
WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximay 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (tri methyl K36) peptide (ab1785). |
ChIP | ChIP: Use 4µg for 106 cells. |
ChIP/Chip | ChIP/Chip: Use at an assay dependent dilution. |
IHC-P | IHC-P: Use a concentration of 0.5 - 10 µg/ml. |
Target
Database links
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Alternative names
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Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade images
ChIP - Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab9050 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH (active) and MYO-D (inactive) promoters and over the ý-Actin gene (active). Schematic diagram of the ý-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)
Staining of human tonsil using ab9050 is shown. Antigen retrieval was performed using Tris EDTA at pH9. Nuclei of lymphoid cells in the interfollicular area of a human tonsil stained strongly positive as well as the endothelial cells of blood vessels.
Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)
ICC/IF image of ab9050 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab9050, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)This image is courtesy of an anonymous Abreview
ab9050 staining rat liver tissue sections by IHC-P. The section was formaldehyde fixed and subjected to heat mediated antigen retrieval in pH 6.0 citrate buffer prior to being blocked with 5% serum for 30 minutes at 45°C. The primary antibody was diluted 1/500 and incubated for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit was used as the secondary
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)This image is courtesy of an anonymous Abreview
ab9050 staining mouse kidney tissue sections by IHC-P. The section was formaldehyde fixed and subjected to heat mediated antigen retrieval in pH 6.0 citrate buffer prior to being blocked with 5% serum for 30 minutes at 20°C. The primary antibody was diluted 1/500 and incubated for 45 minutes at 20°C. A HRP conjugated goat anti-rabbit was used as the secondary
See Abreview
ChIP - Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)This image is courtesy of an anonymous Abreview
ChIP using ab9050 at the Pho4 locus in S. pombe. Chromatin extract from S.pombe cells was incubated with 5 ug of ab9050 overnight, and then incubated with Protein A beads for 1 hour. The immunoprecipitated DNA was quantified at the Pho4 locus by RT-PCR. No antibody was added to the beads control (red bars). A schematic diagram of the pho4 gene is shown below the graph. The grey box represents the open reading frame and PCR products are depicted by horizontal arrows. Please see anonymous abreview submitted on 29 October 2008 for additional details.See Abreview
Western blot - Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)
All lanes : Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) at 1 µg/ml
Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate
Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate with Histone H3 peptide - unmodified K36 at 0.5 µg/ml
Lane 3 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K36) peptide (ab1783) at 0.5 µg/ml
Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K36) peptide (ab1784) at 0.5 µg/ml
Lane 5 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K36) peptide (ab1785) at 0.5 µg/ml
Lane 6 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K37) peptide (ab24417) at 0.5 µg/ml
Lysates/proteins at 0.5 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050)This image is courtesy of an anonymous Abreview
ab9050 staining Histone H3 (tri methyl K36) in Human Saos-2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/1000) for 1 hour. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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Abcam位于英國的劍橋科學園,成立于1998年,專門生產和分銷研究型抗體。主要創立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網上抗體公司,理念是Abcam銷售質量高的抗體,并提供zui全面、誠實、時新的說明書,快速的送貨服務,及高效率的顧客和服務。我們的在線目錄(ladywindows.cn) 已有差不多100,000種抗體和試劑,并不斷添加,供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市,在美國馬薩諸塞州、日本東京及香港均設有分公司。
Abcam 一直致力加強產品線,為使研究員更容易找到蛋白質研究試劑產品,在2011并購了美國的MitoSciences公司,加強了免疫分析方面的產品供應;同年也并購了英國的Ascent Scientific 公司,開展了生化試劑的供應。在2012年,Abcam并購了美國的Epitomics 公司,成為一家有的RabMAbs® 供應商。
Abcam 的 目標是給世界上的抗體建立zui大的在線目錄,為各地科學家提供*產品,成為各國科學界的重要伙伴。我們為所有產品提供來使客戶獲得預期的結 果。為提供高質量的抗體來指向盡可能多的靶蛋白,我們盡所能在盡可能多的應用和物種中檢測每種抗體。我們相信誠信才是上策,在我們的上會發布有關我們 每一種產品的盡可能多的信息。
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