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Anti-Histone H3 (phospho S10) [mAbcam 14955] antibody - ChIP Grade (ab14955)抗組蛋白H3抗體產品信息:
Mouse monoclonal [mAbcam 14955] to Histone H3 (phospho S10) - ChIP Grade
Reacts with: Mouse, Rat, Human, Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), Indian Muntjac, African Green Monkey
Predicted to work with: Chicken, Saccharomyces cerevisiae, Caenorhabditis elegans, Schizosaccharomyces pombe, Zebrafish
Phospho S10 specific clone produced using a synthetic peptide derived from residues 1 - 100 of Human Histone H3, phosphorylated at S10 and tri methylated at K9.
Read Abcam's proprietary immunogen policy
Hybridomas were prepared and the resulting clones were positively screened by ELISA against the immunising tri methyl K9 and phospho S10 dimodified peptide. Clones were also positively screened against both tri methyl K9 and phospho S10 peptides. Clones were negatively screened against the unmodified version of the peptides. This clone binds to the tri methyl K9 and phospho S10 dimodified peptide and to the phospho S10 peptide, but not to the tri methyl K9 peptide or to equivalent unmodified Histone H3 peptide.
Properties
Concentration 100 µg at 1 mg/ml
Applications
Our Abpromise guarantee covers the use of ab14955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
IHC-P | IHC-P: Use at an assay dependent dilution. |
ICC/IF | ICC/IF: Use at an assay dependent dilution. |
ChIP | ChIP: Use at an assay dependent dilution. PubMed: 20864037See Abreview submitted on 5 January 2007. |
IP | IP: Use at 80 µg/mg of lysate. |
IHC-Fr | IHC-Fr: Use at an assay dependent concentration. PubMed: 21734301 |
Flow Cyt | Flow Cyt: 1/1000. |
WB | WB: Use a concentration of 1 - 5 µg/ml. Detects a band of approximay 17 kDa (predicted molecular weight: 15 kDa). |
Target
Database links
Alternative names
Anti-Histone H3 (phospho S10) [mAbcam 14955] antibody - ChIP Grade images
Western blot - Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade (ab14955)
All lanes : Anti-Histone H3 (phospho S10) [mAbcam 14955] antibody - ChIP Grade (ab14955) at 1 µg/ml
Lane 1 : Control HeLa Histone Prep 0.5ug
Lane 2 : Colcemid treated HeLa Histone prep 0.5ug
Lane 3 : Control HeLa Histone Prep 0.5ug with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644) at 1 µg/ml
Lane 4 : Colcemid treated HeLa Histone prep 0.5ug with Human Histone H3 (tri methyl K9, phospho S10) peptide (ab15644) at 1 µg/ml
Lane 5 : Control HeLa Histone Prep 0.5ug with Human Histone H3 (unmodified ) peptide (ab7228) at 1 µg/ml
Lane 6 : Colcemid treated HeLa Histone prep 0.5ug with Human Histone H3 (unmodified ) peptide (ab7228) at 1 µg/ml
Lane 7 : Control HeLa Histone Prep 0.5ug with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml
Lane 8 : Colcemid treated HeLa Histone prep 0.5ug with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml
Lane 9 : Control HeLa Histone Prep 0.5ug with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg/ml
Lane 10 : Colcemid treated HeLa Histone prep 0.5ug with Human Histone H3 (phospho S28) peptide (ab14793) at 1 µg/ml
Secondary
Rabbit polyclonal to Mouse IgG H&L (HRP) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955)This image was submitted as part of a review by Kirk McManus
Indian muntjac and HeLa cells were fixed in paraformaldehyde and labelled with ab14955 (1/2000 dilution) for 30 minutes. The above image contains an interphase and a prophase Indian muntjac cells immunofluorescently labelled with anti-PhosS10 (green) and counterstained with DAPI (red).
ELISA - Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955)
By ELISA, ab14955 detects:
the singly modified phospho S10 peptide and the dual modified phospho S10 and tri methyl K9 peptide (the orange and grey lines respectively),
less strongly detects the phospho S28 peptide (purple line),
does not detect the equivalent non-modified Histone H3 peptide for S10 or the singly modified tri methyl K9 peptide (the 2 lines at the bottom of the figure).
The antibody recognises phospho S28 by ELISA (although a phospho S28 peptide does not block the antibody in Western blotting).
Flow Cytometry - Histone H3 (phospho S10) antibody [mAbcam 14955] - ChIP Grade (ab14955)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab14955 (1/1000) stainning a population of Human HeLa cells positive for Histone H3 (phospho S10). Cells were trypsizined, pelleted and fixed in ice cold ethanol. Cellular debris was eliminated and the FL2-A/FL2-W was used to eliminate clumping cells. For further experimental details please refer to abreview.Western blot - Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955)
Lane 1 - 8 : Histone H3 (phospho S10) antibody [mAbcam 14955] (ab14955) at 0.5 ug/ml
Lane 1 : Control HeLa Histone prep at 5 ug
Lane 2 : Colcemid treated HeLa Histone prep at 5 ug
Lane 3 : Control HeLa Histone prep at 5 ug with Histone H3 peptide - unmodified (ab7228) at 1 ug/ml
Lane 4 : Colcemid treated HeLa Histone prep at 5 ug with Histone H3 peptide - unmodified (ab7228) at 1 ug/ml
Lane 5 : Control HeLa Histone prep at 5 ug with Histone H3 peptide - phospho S28 (ab14793) at 1 ug/ml
Lane 6 : Colcemid treated HeLa Histone prep at 5 ug with Histone H3 peptide - phospho S28 (ab14793) at 1 ug/ml
Lane 7 : Control HeLa Histone prep at 5 ug with Histone H3 peptide - phospho S10 (ab11477) at 1 ug/ml
The antibody is not blocked by a phospho S28 peptide by Western Blotting.
Western blot - Anti-Histone H3 (phospho S10) [mAbcam 14955] antibody - ChIP Grade (ab14955)Image courtesy of Richelle Sopko, Harvard University,U.S.A
All lanes : Anti-Histone H3 (phospho S10) [mAbcam 14955] antibody - ChIP Grade (ab14955) at 1/1000 dilutionImage courtesy of Richelle Sopko, Harvard University, U.S.A
Blocking: 10% BSA
wee shRNA embryos (lane 2) should display elevated phospho H3Ser10 levels relative to wild type
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Abcam位于英國的劍橋科學園,成立于1998年,專門生產和分銷研究型抗體。主要創立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網上抗體公司,理念是Abcam銷售質量高的抗體,并提供zui全面、誠實、時新的說明書,快速的送貨服務,及高效率的顧客和服務。我們的在線目錄(www.abcam.cn) 已有差不多100,000種抗體和試劑,并不斷添加,供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市,在美國馬薩諸塞州、日本東京及香港均設有分公司。
Abcam 一直致力加強產品線,為使研究員更容易找到蛋白質研究試劑產品,在2011并購了美國的MitoSciences公司,加強了免疫分析方面的產品供應;同年也并購了英國的Ascent Scientific 公司,開展了生化試劑的供應。在2012年,Abcam并購了美國的Epitomics 公司,成為一家有的RabMAbs® 供應商。
Abcam 的 目標是給世界上的抗體建立zui大的在線目錄,為各地科學家提供*產品,成為各國科學界的重要伙伴。我們為所有產品提供來使客戶獲得預期的結 果。為提供高質量的抗體來指向盡可能多的靶蛋白,我們盡所能在盡可能多的應用和物種中檢測每種抗體。我們相信誠信才是上策,在我們的上會發布有關我們 每一種產品的盡可能多的信息。
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