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Anti-SATB2 antibody 抗SATB2抗體產品信息:
Rabbit polyclonal to SATB2
Reacts with: Mouse, Rat, Human
Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SATB2.
Read Abcam's proprietary immunogen policy
(Peptide available as ab34906.)
Properties
Concentration 100 µg at 1 mg/ml
Applications
Our Abpromise guarantee covers the use of ab34735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
---|---|
WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximay 85 kDa (predicted molecular weight: 83 kDa).Can be blocked with Mouse SATB2 peptide (ab34906). |
EMSA | EMSA: Use at an assay dependent concentration. PubMed: 22123820 |
IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. |
ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 19232401 |
Target
Alternative names
see all
Anti-SATB2 antibody images
Western blot - SATB2 antibody (ab34735)
Anti-SATB2 antibody (ab34735) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 83 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Electrophoretic Mobility Shift Assay - Anti-SATB2 antibody (ab34735)Image from Asanoma K et al, J Biol Chem. 2012 Jan 13;287(3):2257-68. Epub 2011 Nov 28, Fig 8. DOI 10.1074/jbc.M111.287128 January 13, 2012 The Journal of Biological Chemistry, 287, 2257-2268.
ab34735 used in EMSA.
EMSA demonstrating binding of SATB1 and SATB2 to an oligonucleotide containing a predicted SATB-binding element located 125 bp upstream of the Eomes transcription start site (-125). Labeled SATB probes were incubated with Rcho-1 TS cell nuclear lysates as indicated above the lanes. Lane 1, without any competitor oligonucleotide; lane 2, with unlabeled wild-type competitor; lane 3, with unlabeled mutant competitor; lane 4, with SATB1 antibody; lane 5, with SATB2 antibody (ab34735); lane 6, with a control antibody. B and C, Rcho-1 TS cells stably transfected with pcDNA3, pcDNA3-HA-Satb1, or pcDNA3-HA-Satb2 and maintained in stem culture conditions were used for ChIP assays. Antibodies to the HA tag (Anti-HA) or acetylated histone H3K9 (Anti-AcH3) were used in the analyses. IgG was used as a control for nonspecific immunoprecipitation. qPCR analyses were performed on immunoprecipitates using primers amplifying the segment (-172 to -63) including the SATB-binding element at -125.
Immunocytochemistry/ Immunofluorescence - Anti-SATB2 antibody (ab34735)Image courtesy of an anonymous Abreview.
ab34735 staining SATB2 in human osteosarcoma SAOS-2 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using PBS/ 0.25% Triton, blocked with 1% BSA for 1 hour at room temperature and then incubated with ab34735 at a 1/500 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/250 dilution.
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Abcamab11375Rat monoclonal [YTH 8.18] to Macrophages+Neutrophils250ul3996Macrophage/Neutrophilshystemcell一抗
Abcamab11379Mouse monoclonal [8E9] to Glucocorticoid Receptor100ug3996Glucocorticoid Receptorhystemcell一抗
Abcamab1138Rabbit polyclonal to GRC5/PHF2100ul3996GRC5/PHF2hystemcell一抗
Abcamab11380Mouse monoclonal [MAB 263] to Growth hormone receptor100ug3996Growth hormone receptorhystemcell一抗
Abcamab11382Mouse monoclonal [INN-hCG-106] to hCG beta core fragment250ug3996hCG beta core fragmenthystemcell一抗
Abcamab11386Mouse monoclonal [INN-hFSH-158] to Glycoprotein hormones (alpha 5)250ug3996Glycoprotein hormones (alpha 5)hystemcell一抗
Abcamab11387Mouse monoclonal [INN-hCG-72] to hCG alpha 6250ug3996hCG alpha 6hystemcell一抗
Abcamab11388Mouse monoclonal [INN-hCG-2] to hCG beta 1 epitope250ug3996hCG beta 1hystemcell一抗
Abcamab11389Mouse monoclonal [INN-hCG-22] to hCG beta 2 epitope250ug3996hCG beta 2hystemcell一抗
Abcamab1139Rabbit polyclonal to HAP40100ul3996HAP40hystemcell一抗
Abcamab11390Mouse monoclonal [ INN-hCG-24 ] to hCG beta 4 epitope250ug3996hCG beta 4hystemcell一抗
Abcamab11391Mouse monoclonal [ INN-hCG-68 ] to hCG beta 7250ug3996hCG beta 7hystemcell一抗
Abcamab11392Mouse monoclonal [INN-hCG-53] to hCG Holo c2250ug3996hCG Holo c2hystemcell一抗
Abcamab11393Mouse monoclonal [INN-hCG-45] to hCG Holo c3250ug3996hCG Holo c3hystemcell一抗
Abcamab11396Mouse monoclonal [INN-hPL-37] to Human placental lactogen (epitope 2)250ug3996placental lactogenhystemcell一抗
Abcamab11399Rat monoclonal [ICR61] to EGFR100ug3996EGFRhystemcell一抗
Abcamab114Rabbit polyclonal to LIMPII100ul3996Scavenging Receptor SR-BIIhystemcell一抗
Abcamab1140Rabbit polyclonal to Cyclin E100ul3996Cyclin E1hystemcell一抗
Abcamab11400Rat monoclonal [ICR10] to EGFR (FITC)100ug5029EGFRhystemcell一抗
Abcamab11401Mouse monoclonal [F4] to EGFR (FITC)500ul3996EGFRhystemcell一抗
Abcamab11402Mouse monoclonal [H44] to IL18R1100ug3996IL-18R1hystemcell一抗
Abcamab11404Mouse monoclonal [H44] to IL18R1 (Biotin)50ug3996IL-18R1hystemcell一抗
Abcamab11408Mouse monoclonal [G16] to Interferon alpha125ug3996IFN-alphahystemcell一抗
Abcamab11409Sheep polyclonal to Interferon beta50ul3996IFN-betahystemcell一抗
Abcamab1141Rabbit polyclonal to Cyclin E2100ul3996Cyclin E2hystemcell一抗
Abcamab11411Mouse monoclonal [LMM775 ] to GCSF Receptor (Phycoerythrin)50tests3996G-CSF Receptorhystemcell一抗
Abcamab11412Mouse monoclonal [D9D10] to Interferon gamma250ug3996IFN-gammahystemcell一抗
Abcamab11413Mouse monoclonal [B27] to Interferon gamma (Allophycocyanin)50tests3996IFN-gammahystemcell一抗
Abcamab11414Mouse monoclonal [SN6] to CD105100ug3996CD105hystemcell一抗
Abcamab11415Mouse monoclonal [SN6] to CD105 (FITC)100ug5029CD105hystemcell一抗
Abcamab11417Mouse monoclonal [D9D10] to Interferon gamma (FITC)50ug3996IFN-gammahystemcell一抗
Abcamab11418Mouse monoclonal [Z-ZE4] to IGFBP150ug3996IGFBP1hystemcell一抗
Abcamab11419Mouse monoclonal [9C8] to GADD15350ug4418GADD153hystemcell一抗
Abcamab1142Rabbit polyclonal to Bcl10100ul3996Bcl-1hystemcell一抗
Abcamab11420Rabbit polyclonal to ERp2950ul3996ERp29hystemcell一抗
Abcamab11421Rabbit polyclonal to ERp5750ul3996ERp57hystemcell一抗
Abcamab11422Rabbit polyclonal to ERp7250ug3996ERp72hystemcell一抗
Abcamab11423Mouse monoclonal [1234] to Rapsyn50ug3996Rapsynhystemcell一抗
Abcamab11424Rabbit polyclonal to EDG150ug3996EDG1hystemcell一抗
Abcamab11425Mouse monoclonal [1351] to Syntrophin50ug3996Syntrophinhystemcell一抗
更多詳情,請:
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