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Anti-Ctip2 antibody 25B6 - ChIP Grade

簡要描述:Anti-Ctip2 [25B6] antibody - ChIP Grade
貨號:ab18465
廠家:Abcam公司
規格:100ul
保存溫度:-20℃ 和 2~8℃
貨期:現貨2~5天,期貨2-3周

  • 產品型號:ab18465
  • 廠商性質:代理商
  • 更新時間:2017-01-25
  • 訪  問  量:1739
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 咨詢:  (重慶) (貴陽)

Anti-Ctip2 antibody [25B6] - ChIP Grade產品信息:

  • Product nameAnti-Ctip2 antibody [25B6] - ChIP Grade
  • Description

    Rat monoclonal [25B6] to Ctip2 - ChIP Grade

  • SpecificityDetects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Tested applicationsICC/IF, IHC-FoFr, WB, IP, IHC-P, IHC-Fr, ChIP, IHC-FrFl, Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Guinea pig, Human, Zebrafish

  • Immunogen

    Fusion protein (Human).

  • EpitopeBetween amino acids 1-150 of CTIP2.
  • Positive control
    • WB: nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody IHC: adult mouse hippocampus, Adult mouse spinal cord ICC: Neonatal mouse hippocampal cultured neurons
  • General notesHybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 150mM Sodium chloride, 15mM HEPES, pH 7.5
  • Concentration100 µg at 1 mg/ml 

  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number25B6
  • IsotypeIgG2a
  • ApplicationNotes
    ICC/IFICC/IF: 1/500.
    IHC-FoFrIHC-FoFr: Use at an assay dependent dilution. PubMed: 18523013
    WBWB: Use at an assay dependent dilution. Detects a band of approximay 120 kDa (predicted molecular weight: 95 kDa).
    IPIP: Use at an assay dependent dilution.
    IHC-PIHC-P: 1/500.
    IHC-FrIHC-Fr: 1/500.
    ChIPChIP: Use at an assay dependent dilution.
    IHC-FrFlIHC-FrFl: Use at an assay dependent concentration.
    Flow CytFlow Cyt: Use 1µg for 106 cells.

    Target

  • FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Anti-Ctip2 antibody [25B6] - ChIP Grade images

  • Immunohistochemistry (frozen sections) - ctip2 antibody [25b6] - chip grade (ab18465)

    Immunohistochemistry (Frozen sections) - Ctip2 antibody [25B6] - ChIP Grade (ab18465)This image is courtesy of an anonymous Abreview

    ab18465 staining Ctip2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG (1/1000) was used as secondary antibody. Staining was improved with citrate antigen retrieval.
  • Immunocytochemistry/ immunofluorescence - ctip2 antibody [25b6] - chip grade (ab18465)

    Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

    Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

    Red is beta tubulin staining.
    Green is ab18465.

  • Immunocytochemistry - anti-ctip2 antibody [25b6] - chip grade (ab18465)

    Immunocytochemistry - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

    Immunohistochemistry using ab18465 on Adult Mouse Hippocampus.

  • Immunocytochemistry/ immunofluorescence - ctip2 antibody [25b6] - chip grade (ab18465)

    Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

    Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.

  • Western blot - ctip2 antibody [25b6] - chip grade (ab18465)

    Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)
    Predicted band size : 95 kDa
    Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

  • Western blot - ctip2 antibody [25b6] - chip grade (ab18465)

    Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)

    All lanes : Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) at 1/500 dilution

    Lane 1 : Mouse brain tissue lysate at 1.5 µg
    Lane 2 : Mouse brain tissue lysate at 1.5 µg
    Lane 3 : Mouse brain tissue lysate at 3 µg

    Secondary
    IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 95 kDa
    Observed band size : 100,110 kDa


    Exposure time : 10 minutes

    This image is courtesy of an Anonymous Abreview.

  • Immunohistochemistry (formalin/pfa-fixed paraffin-embedded sections) - anti-ctip2 antibody [25b6] - chip grade (ab18465)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)

    ab18465 staining Ctip2 in Guinea Pig Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
  • Flow Cytometry-Anti-Ctip2 antibody [25B6] - ChIP Grade(ab18465)

    Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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